Amperometric glucose biosensors based on layer-by-layer assembly of chitosan and glucose oxidase on the Prussian blue-modified gold electrode
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Abstract: A glucose biosensor based on layer-by-layer (LBL) self-assembling of chitosan and glucose oxidase (GOD) on a Prussian blue film was developed. First, Prussian blue was deposited on a cleaned gold electrode then chitosan and GOD were assembled alternately to construct a multilayer film. The resulting amperometric glucose biosensor exhibited a fast response time (within 10 s) and a linear calibration range from 6 μM to 1.6 mM with a detection limit of 3.1 μM glucose (s/n = 3). With the low operating potential, the biosensor showed little interference to the possible interferents, including ascorbic acid, acetaminophen and uric acid, indicating an excellent selectivity.
Keywords Chitosan - Glucose biosensor - Glucose oxidase - Layer-by-layer assembly - Prussian blue
Biotechnol Lett (2008) 30:317–322
DOI 10.1007/s10529-007-9534-z
Received: 21 May 2007 / Revised: 3 September 2007 / Accepted: 3 September 2007 / Published online: 3 October 2007
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Mcdull 在2008-12-1 17:26 评分: Vip +1.222 原因:
Thx for your application for qualified member and please read the thread at http://chem8.org/bbs/thread-20198-1-1.html. If interested, could you share with us the story of this paper at http://chem8.org/bbs/forum-46-1.html?Thx!
Mcdull 在2008-12-1 17:26 评分: 金币 +100 原因:
Thx for your application for qualified member and please read the thread at http://chem8.org/bbs/thread-20198-1-1.html. If interested, could you share with us the story of this paper at http://chem8.org/bbs/forum-46-1.html?Thx!